Canale - Parola and S B Leschine complexes of Clostridium papyrosolvens C 7 . Ultrastructural diversity of the cellulase
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Cellulase system of a free-living, mesophilic clostridium (strain C7).
The enzymatic activity responsible for crystalline cellulose degradation (Avicelase activity) by a mesophilic clostridium (strain C7) was present in culture supernatant fluid but was not detected in significant amounts in association with whole cells or in disrupted cells. Cells of the mesophilic clostridium lacked cellulosome clusters on their surface and did not adhere to cellulose fibers. Th...
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Suringar (1866) and Smit (1930) reported that cell packets of Sarcina ventriculi exhibited a positive cellulose reaction (stained red to purple) when suspended in an aqueous solution containing ZnC12, 12, and KI. These workers believed that cellulose was localized in the cell wall. On the other hand, Delaporte (1957) could not obtain this color reaction and reported that examination of S. ventr...
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Study of Sarcina ventriculi (Zymosarcina ventriculi) has been encumbered by the absence of a satisfactory stock culture method. Smit (1930), as well as Milhaud et al. (1956) reported that transfers of the organism at 1or 2-day intervals were necessary to keep viable cultures. In this paper a method is described in which a viable stock culture may be maintained for a period of 2 months. A brief ...
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